大卫E. 滚,Ph值.D
Professor Emeritus of Organic 化学istry, 物化学istry, and Human 医学杂志ogy教育
伊利诺伊州厄巴纳博士.D. 生物化学(1976)
伊利诺伊大学.S. 生物化学(1972)
哈丁学院B.S. (1970)化学(阿肯色州Searcy)
传记
关于博士. 卷
我出生在马里兰州的巴尔的摩. My parents decided to relocate to the Midwest so I decided to move with them. I grew up in Kansas City Missouri and then attended Harding University in 搜索 Arkansas where I completed my B.S. 化学学位. I completed my Masters degree and PhD degree in biochemistry at the University of Illinois Urbana. I studied the structures of complex carbohydrates on the plasma membrane and inside the cells of chick fibroblasts infected with Rous Sarcoma virus. 研究生学习提供了另一个好处. While working in the laboratory I met my future wife Peggy and we had the right chemistry. With five children and two grandchildren we are still happily married. We moved to the University of Pittsburgh School of Medicine where I studied the disease of alpha-1-antitrypsin deficiency. I started teaching in the chemistry department at Roberts Wesleyan College after completing my postdoctoral work. I am interested in the synthesis and regulation of topoisomerase enzymes, 尤其是衣原体细菌, and the efficient purification of alpha-1-antitrypsin from human serum and calf serum. In addition I am pursuing research projects in forensic science involving DNA identity testing. I have also completed Sabbatical research projects at the Baylor College of Medicine, 罗彻斯特大学医学院, and the Center for Fluorescence Spectroscopy at University of Maryland School of Medicine.
社会兼职
Alpha Chi Sigma化学兄弟会
美国科学协会
美国生物化学与分子生物学学会
全国卫生专业顾问协会
美国科学家联合会
本科生研究委员会
研究兴趣
真核细胞分化, 动物细胞膜和细胞表面的碳水化合物, 糖蛋白的合成和分泌, 病毒细胞转化, 拓扑异构酶与细胞转化, 衣原体拓扑异构酶基因.
赠款资助
重组DNA技术在本科课程中的应用,美国国家科学基金会, 大学科学仪器项目, 配对补助金$6,000, NSF; $6,000, RWC (1985).
高等本科生细胞生物学实验室,美国国家科学基金会, 大学科学仪器项目, 配对补助金$11,000, NSF; $11,000, RWC, (1991).
"Identification of Possible New 化学otherapeutic Targets in Chlamydia,“学术研究促进奖”, 美国国立卫生研究院, $93,610, (1996).
在本科课程中介绍FT-NMR,美国国家科学基金会, CCLI适应与实施方案, 配对补助金$27,187.50, (2000).
专利
Coinventors: David 卷, Ignacy Grczynski, Joanna Malicka, Zygmunt Gryczynski and Joseph R. Lakowicz. 专利 Disclosure JRL-63 金属胶体波长比率散射传感器”
出版物及摘要
韦伯,米.J. 黑尔,R.H.和滚,维.E., "Role of Protease Activity in Malignant Transformation by Rous Sarcoma Virus,蛋白酶和生物控制, pp. 915-930，冷泉港实验室，1975.
滚,维.E. 康拉德，H.E., "Quantitative Radiochromatographic Analysis of the Major Groups of Carbohydrates in Cultured Animal Cells,“肛门. 物化学., 77, 397-412 (1977).
滚,维.E.[J].咖啡，C.J. 和辉光，R.H., "Comparison of the Carbohydrate and Amino Acid Composition of Normal and S-Variant Alpha-1-Antitrypsins,“生物化学. Biophys. 学报，532,171-178 (1978).
伦纳德,J.黑尔，A.D, 卷, 卷。.康拉德，H.E. 韦伯，M。., "Turnover of Cellular Carbohydrates in Normal and Rous Sarcoma Virus-Transformed Cells,癌症研究, 38, 185-188 (1978).
滚,维.E.[J]. 和辉光，R.H., "Studies on the Attachment of N-Acetylneuraminic Acid to Glycopeptides from Alpha-1-Antitrypsin,“美联储. Proc. 37、摘要#1828 (1978).
滚,维.E.[J].咖啡，C.J.发光，R.H., "Isolation and Characterization of Cyanogen Bromide Derived Glycopeptide Fragments of Alpha-1-Antitrypsin," J. 医学杂志. 化学., 253, 6992-6996 (1978).
Aguanno J.D, 卷, 卷。.E.咖啡，C.J.发光，R.H., "Studies on the Attachment of N-Acetylneuraminic Acid to Glycopeptides from Alpha-1-Antitrypsin," J. 医学杂志. 化学., 253, 6997-7004 (1978).
滚,维.E.韦伯，M。.J.康拉德，H.E., "Effect of Growth Conditions on the Content of the Major Groups of Carbohydrates in Chick Embryo Fibroblasts,癌症研究, 39, 2550-2555 (1979).
德班,E.D, 卷, 卷。.贝克纳，G. 布什，H., "Purification and Characterization of the Nuclear DNA-Binding Phosphoprotein 110/8.4在肿瘤和胎儿组织中发现. Proc. 39、摘要#1856 (1980).
德班,E.D, 卷, 卷。.贝克纳，G.布什，H., "Purification and Characterization of a Nuclear DNA-binding Phosphoprotein in Fetal and Tumor Tissues,癌症研究, 41, 537-545 (1981).
滚,维.E.发光，R.H., "Isolation and Characterization of Rat Alpha-1-Antitrypsin," J. 医学杂志. 化学. 256, 8190-8196 (1981).
德班,E.米尔斯，南卡罗来纳州.D, 卷, 卷。. 布什，H., "Phosphorylation of Purified Hepatoma Topoisomerase I,“生物化学. Biophys, Res. 通讯., 111, 897-905 (1983).
饶,M.R.S.， Ahr, V.S.斯宾恩，W.H.德班，E.D, 卷, 卷。. 布什，H., "A Novel Acidic (p14) 19 Kilodalton Nucleolar Phosphoprotein," J. Cell 医学杂志ogy, 97, American Society for Cell 医学杂志ogy Abstract #527, 140a (1983).
McRorie D.K.饶,M.R.S.戈尔德克诺夫，我.L.哈蒂，T.P.D, 卷, 卷。.E.安，V.S.布什，H.19KD/pI 4的纯化及部分表征.5 .核仁磷酸化蛋白，《生物化学. Biophys, Res. 通讯., 122, 47-55 (1984).
滚,维. 和德班，E., "The Topoisomerase I Tyrosine Phosphorylation Site and the DNA-Interactive Site,美国生物化学家学会. 摘要#1707，(1986).
滚,维.，“血红蛋白功能模拟”软件综述[J]. 化学. Ed., 65, A113-114 (1988).
滚,维., 重组DNA技术在本科课程中的应用," abstract and poster submitted to Conference on Gene Splicing sponsored by the 美国科学协会, 7月, 1988.
Bramucci, M.D, 卷, 卷。.库克，R。. 和德班，E., "Identification of Consensus Epitope Structures Expressed in Recombinant DNA Libraries,分子免疫学, 26, 741-748 (1989).
滚,维.墨菲，B.J.雷德路特，K.R.萨瑟兰，R.M. 史密斯，H.C., "Oxygen Regulated 80KDa Protein and Glucose Regulated 78KDa Protein Are Identical,分子和细胞生物化学, 103, 141-148 (1991).
Daschev C.D, 卷, 卷。.E.巴伐利亚，P.M., "Expression and Translocation of the Chlamydial Major Outer Membrane Protein in Escherichia coli,微生物发病机制, 15, (1993).
滚,维.夏，R.巴伐利亚，P.M., "Optimization of Expression and Translocation of the Chlamydial Porin in Escherichia coli." In "Proceedings at the Eighth International Symposium on Human Chlamydial Infection," Orfilia et. al.(eds.)，《意大利社会杂志》，博洛尼亚，263-266 (1994).
亚当斯,R.D, 卷, 卷。., "Isolation of a Partial Topoisomerase I Gene Clone from the GPIC strain of Chlamydia Psittaci." Abstract and poster presented at the Ninth International Symposium on Human Chlamydial Infection, 6月21-26, 1998, 纳帕谷, 加州.
戈德斯,C. D.帕尔菲诺夫，A. Gryczynski,我.玛丽卡，J.D, 卷, 卷。.拉科维茨，J. R., “Fractal Silver Structures for Metal-Enhanced Fluorescence: Applications for Ultra-Bright Surface Assays and Lab-on-a-Chip-Based Nanotechnologies,荧光杂志, 13(2), 123-128, 2003.
滚,维.玛丽卡，J.格里琴斯基，我.格里琴斯基，Z.拉科维茨，J. R., 金属胶体波长比率散射传感器,分析化学75(14), 3440-3445 (2003).
Lakowicz J.R.玛丽卡，J.格里琴斯基，Z.D, 卷, 卷。.黄，J.格迪斯，C. D.格里琴斯基，我. , “Increased Sensitivity of Fluorescence Detection Using Metallic Nanoparticles,“PharmaGenomics, 3(3), 38-46 (2003).
戈德斯,C.D.帕尔菲诺夫，A.D, 卷, 卷。.Fang, J.拉科维茨，J.R., “Electrochemical and Laser Deposition of Silver for Use in Metal-Enhanced Fluorescence,Langmuir 19(15), 6236-6241 (2003).
戈德斯,C.D.帕尔菲诺夫，A.D, 卷, 卷。. Gryczynski,我.玛丽卡，J.拉科维茨，J. R., “Silver Fractal-like Structures for Metal-enhanced Fluorescence: Enhanced Fluorescence Intensities and Increased Probe Photostabilities,《美高梅mgm平台》13(3), 267-276 (2003).
戈德斯,C. D.D, 卷, 卷。.帕尔菲诺夫，A.拉科维茨，J. R., 用于金属增强荧光的贵金属表面,海报#B705, presented at the 47th Annual Meeting of the Biophysical Society, 圣安东尼奥, 德州, 3月1 - 5, 2003.
戈德斯,C. D.帕尔菲诺夫，A.罗德，D.拉科维茨，J. R., “Enhanced Photostability of Indocyanine Green on a Roughened Platinum Electrode: Metal-enhanced Fluorescence,《美高梅mgm平台》13(6), 453-457 (2003).
戈德斯C. D.帕尔菲诺夫，A.D, 卷, 卷。.拉科维茨，J. R., “Roughened Silver Electrodes for Use in Metal-enhanced Fluorescence,” Spectrochemica Acta A., 60 (8-9), 1977-1983, (2004).
张,我.D, 卷, 卷。.格迪斯，C. D.拉科维茨，J. R., “Aggregation of Silver Nanoparticle-Dextran Adducts with Concanavalin A and Competitive Displacement with Glucose,物理化学学报B, 108 (32), 12210-12214 (2004).